Phytoplasma
Plant parasitic microorganism Phytoplasma
It can cause you trouble when your hydrangea does not color like ordinary years, or stays green even though the flowers are in bloom. In such case, your plant could have a disease called hydrangea phyllody disease, caused by Phytoplasma.
Phytoplasma disease, including hydrangea phyllody disease, is caused by microorganism called “Phytoplasma” parasitic on plants. This microorganism is transmitted by insects such as leafhoppers and is also infected by vegetative propagation such as grafting.
Typical examples that caused by Phytoplasma are Hydrangea phyllody disease that affects hydrangea, and witches’ broom that affects sweet potatoes.
In tropical regions, Phytoplasma has become problematic by infecting coconut palms dying from Phytoplasma disease that has severe effects on people’s daily life.
If a plant is infected with phytoplasma disease, it will die within a few years, and if left untreated, the infection will spread around the infected plant.
Therefore, control measures such as removal of infected plants are important.
Nippon Gene has a lineup of kits that offer the detection of phytoplasma with simple diagnostic techniques.
Plant parasitic microorganism Phytoplasma
- What is it: A microorganism that infects hydrangea and sweet potatoes
- What happens: Causes various symptoms on plant when infected
- What if you don’t disinfect early: Infection will spread through insects
Hydrangea Infected with hydrangea phyllody disease Caused by Phytoplasma
Phytoplasma is a phytopathogenic bacterium that parasitizes the phloem of plants, and was first discovered in Japan in 1967 in Japan. Over 700 types of plants in the world and about 80 types of plants in Japan are infected, causing malformation symptoms such as yellowing, dwarf, crowding, penetration, phyllody, and witches’ broom. Phytoplasma is transmitted through insect vectors, vegetative growth of grafting, etc., and an extremely important plant pathogen for agriculture.
In order to prevent the spread of damage caused by phytoplasma, it is necessary to take control measures such as removal of infected plants.
For that purpose, early detection of diseased plants is indispensable, but because phytoplasma is a microorganism that is difficult to culture, there were no simple diagnostic technique established.
Nippon Gene offer “Phytoplasma Universal Detection Kit”. By using the Phytoplasma Universal Detection Kit, it is possible to comprehensively detect phytoplasma from your plant with simple procedure.
Detection Kit
Phytoplasma Universal Detection Kit
A diagnostic kit that comprehensively detects phytoplasma.
* The linked page may be in Japanese, please use the translation function of the browser.
Detection Kit
DryADD™ Phytoplasma Universal Detection LAMP Kit
This product is a dry reagent version of Phytoplasma Universal Detection Kit.
Nippon Gene’s reagent drying technology has made it possible to transport and store the kit at room temperature, to supply and use it in all parts of the world.
LAMP method is employed for sample DNA amplification, and comprehensive detection of phytoplasma belonging to the class Mollicutes is possible.
Features
Shipping and long-term storage* at room temperature are possible
*Original performance (after manufacturing) is maintained after long-term storage at 25 ° C or 45 ° C (stable as frozen-stored liquid reagent)
Packaged individually for each test and can be used immediately after dissolution
Preformulated for reducing contamination risk and human error during reaction mixture preparation
Price
| Product name | Packing unit | Price |
|---|---|---|
| DryADD™ Phytoplasma Universal Detection LAMP Kit | 48 tests | please inquire |
DryADD™ Phytoplasma Universal Detection LAMP Kit
| Component | Qty (48 tests) | Storage |
|---|---|---|
| Phytoplasma Detection Dry Reagent | 8 well × 6 strips |
Room Temperature |
| Phytoplasma Reagent Dissolve Solution | 400 μl × 6 tubes | |
| Phytoplasma Extraction Solution | 1 ml × 48 tubes | |
| Phytoplasma Positive Control | 6 tubes | |
| Phytoplasma Control Dissolve Solution | 400 μl × 1 tube | |
| Phytoplasma Negative Control | 6 tubes | |
| Mineral oil | 400 μl × 6 tubes |
Shipping temperature
This product is shipped at room temperature.
Instructions
* Please carry out each process in the order of A→B→C, do not proceed the process simultaneously
<Step A: Preparation of a DNA sample>
A-1. Take out the required number of Phytoplasma Extraction Solution tube.
A-2. Scale 0.005 g of Coconut wood chip from stem and soak it completely in Phytoplasma Extraction Solution.
A-3. Invert to mix upside down, then incubate the microtube at 95˚C for 10 minutes.
A-4. Invert to mix upside down, then cool with water to room temperature (the solution obtained is DNA sample).
＜Step B: Preparation of positive control＞
B-1. Take out one of Phytoplasma Positive Control tube.
B-2. Add 10 µl of Phytoplasma Control Dissolve Solution.
B-3. After spinning down, leave it at room temperature for 5 minutes.
B-4. After vortexing, spin down the tubes (Phytoplasma Positive Control Solution).
＜Step C: Preparation of test solution and LAMP reaction＞
C-1. Take out the required number of Phytoplasma Detection Dry Reagent.
C-2. Add 23 µl of Phytoplasma Reagent Dissolve Solution to each tube.
C-3. Add 2 µl of sample, close the cap, and spin down.
C-4. After 2 minutes, overlay with 20 µl of Mineral Oil.
*When using a thermal cycler equipped with hot bonnet, it is not necessary to add mineral oil.
C-5. Incubate test tube at 64˚C for 60 minutes (test reaction).
C-6. Terminate the reaction at 80˚C for 2 minutes.
C-7. Make a judgment.
YouTube: Introduction of “LAMP Dried-Premix Phytoplasma Universal Detection Kit”
https://www.youtube.com/watch?v=WVm9iaTZXv0
Remarks
This product is a reagent only for testing and research use. Do not use for medical applications.
License
The LAMP method is patented by Eiken Chemical Co., Ltd. Nippon Gene Co., Ltd. is licensed to develop, manufacture, and commercialize Phytoplasma detection kits using the LAMP method.
The product name has been changed from former “-LAMP Dry Premix- Phytoplasma Universal Detection Kit”, while there is no change in the product content.
Paid sample containing 8 testings is available for trial use.If you would like to test the sample, please contact us.
Detection Kit
Phytoplasma Primer Set
A primer set for LAMP method that detects Ca. P. japonicum or Ca. P. asteris from phytoplasma specifically.
* The linked page may be in Japanese, please use the translation function of the browser.
External link
