Soil Testing
Improving the fertility of soil is essential for growing crops. Soil testing is an important method to know the fertility condition of the soil. Speaking of soil testing, many people think of chemical analyses such as acidity (pH), electrical conductivity (EC), phosphoric acid and nitrogen determination, but in addition to these tests, physical and biological analysis can be also performed and considered very effective.
Various organisms are active in the soil, and there may be a mixture of things that causes plant disease. Therefore, the biological test to check if the cause of the plant disease exists in the soil is very effective to prevent the plant disease.
Nippon Gene provides a biological analysis service by genetic testing for your quick outcome for soil condition.
Example: Clubroot
- What is it: Diseases peculiar to cruciferous plants
- What characteristics: Disperses in soil and survives for years
- What can we do: Check the density of pathogenic bacteria in the soil to apply the optimal control method
Clubroot is a plant disease peculiar to cruciferous plants and is a soil-borne disease that is difficult to control once plant develop it. When infected with clubroot, many small and large bumps form in the roots. Absorption of water and nutrients from the soil is hindered, and the stems and leaves will wither during sunny days. Delayed growth causes the leaf color to fade and the quality of the harvest decreases.
The pathogen (Plasmodiophora brassicae) is a type of mold that exists in the gall of the roots. If the gall rots, it will disperse in the soil and survive without the host plant for more than 4 years, sometimes more than 10 years. For this reason, once a field has been corrupted by infection, damage may occur over a long period of time, and it often spreads from the infected field to the surrounding area due to soil movement or rain during farming.
When judging the application of fungicides as a countermeasure for clubroot, it is effective to know the bacterial density in the soil in advance.
Detection Kit
DryADD™ Clubroot Disease Detection LAMP kit
This dry kit is made for customers who wish to execute the LAMP method for measuring the density of clubroot, which is the same reagent used in the “Plasmodiophora quantification (clubroot testing) service”. This product can be useful for the effective control of clubroot that cause enormous damage in cruciferous vegetable cultivation.
Features
Shipping and long-term storage* at room temperature are possible
*Original performance (after manufacturing) is maintained after long-term storage at 25 ° C or 45 ° C (stable as frozen-stored liquid reagent)
Packaged individually for each test and can be used immediately after dissolution
Preformulated for reducing contamination risk and human error during reaction mixture preparation
| Product name | Packing unit | Price |
| DryADD™ Clubroot Disease Detection LAMP Kit | 48 tests | please inquire |
DryADD™ Clubroot Disease Detection LAMP Kit
| Component | Qty (48 tests) | Storage |
| Plasmodiophora Detection Dry Reagent | 8 well × 6 strips | Room Temperature |
| Plasmodiophora Positive Control | 1 tube | Room Temperature |
| Deionized Distilled Water | 1,200 μl × 3 tubes | Room Temperature |
| Mineral oil | 1,400 μl × 1 tube | Room Temperature |
Shipping temperature
This product is shipped at room temperature.
Note
- To measure (quantify) the clubroot pathogen, a turbidity measurement device is required; please obtain one separately.
- ISOSPIN Fecal DNA (Nippon Gene Co., Ltd.) is also separately required as a nucleic acid extraction reagent for extracting clubroot pathogen DNA from soil.
Overview of testing
Remarks
This product is a reagent only for testing and research use. Do not use for medical applications.
This product utilizes the results of joint research with the Laboratory of Plant Pathology, The University of Tokyo, in the title of “Development of dried reagents that enable long-term room temperature storage of plant disease gene diagnosis kits” adopted by the Toyama Prefecture FY 2016 Industry-Academia-Government Collaboration Promotion Project.
License
The LAMP method is patented by Eiken Chemical Co., Ltd. Nippon Gene Co., Ltd. is licensed to develop, manufacture, and commercialize cassava begomovirus detection kits using the LAMP method.
The product name has been changed from former “Clubroot disease detection kit (LAMP Dry reagents)”, while there is no change in the product content.
Detection Service
Plasmodiophora Quantification (Clubroot Testing) Service
A service to measure the density of Plasmodiophora brassicae from the soil that you provide.
* The linked page may be in Japanese, please use the translation function of the browser.
Soil nematode
- What is it: Also called “roundworm”, many types of nematodes present in soil
- What characteristics: “Plant parasitic nematodes” damages crops
- What can we do: Characterize the type of nematodes in the soil in order to apply the optimal control method
There are many types of nematodes in the soil. While there are nematodes which decompose bacteria, mold, spoilage organic matter, etc. in the soil and do not affect our lives, there are also “plant parasitic nematodes” in the soil that infest plants and damage crops.
Nippon Gene provides a testing service which detects and discriminates almost all of the plant parasitic nematodes “root lesion nematode” (9 species) and “root knot nematode” (4 species) distributed in fields.
Detectable nematode species
Pratylenchus sp. (root lesion nematode) 9 species
・P. coffeae
・P. kumamotoensis
・P. neglectus
・P. pseudocoffeae
・P. crenatus
・P. zeae
・P. loosi
・P. penetrans
・P. vulnus
Meloidogyne sp. (root knot nematode) 4 species
・M. arenaria
・M. hapla
・M. incognita
・M. javanica
Detection Service
Soil Nematode Detection Service
A service to check the presence of plant parasitic nematodes from the soil that you provide.
* The linked page may be in Japanese, please use the translation function of the browser.
External link
