| Product Name | Code No. | Packaging Unit | Price |
| Red tide causative species Detection Kit 2 –Chattonella spp.– | NE171 | 24 tests | inquiry |
Red tide causative species Detection Kit 2 –Chattonella spp.– is designed to detect Red tide causative planktons “Chattonella spp.” using the Loop-mediated Isothermal Amplification (LAMP) method. Chattonella spp. are identified by amplification of a part of their genomic DNA.
LAMP is fast and easy DNA amplification method which is also used for the diagnosis of influenza virus and detection of other infectious pathogens, exhibiting excellent specificity and sensitivity.
These kits enable all reaction processes to progress at a constant temperature with a simple container such as a Thermos pot.
Chattonella is a genus of phytoplankton belonging to the class Raphidophyceae; these organisms can undergo massive blooms, resulting in red tides. While hundreds of phytoplankton species inhabit Japanese waters, approximately twenty kinds bring serious damage to the fisheries industry. Chattonella is one group of these harmful species and poses a risk of causing mass mortality among fish and shellfish even at low population densities.
To prevent significant damage to the fisheries industry, it is crucial to monitor for red tide outbreaks at an early stage and implement countermeasures such as relocating fish pens or suspending feeding.
Traditionally, the identification and density measurement of harmful species have relied primarily on microscopic observation—a process requiring specialized expertise and, at times, extensive labor. This kit enables the simple, highly sensitive, and rapid detection of the highly harmful Chattonella spp.. The species and varieties detectable by this kit include Chattonella marina and its variants, Chattonella marina var. antiqua and Chattonella marina var. ovata.
| Component | Qty (24 tests) | Storage |
| Detection Reagent A | 500 μL | -20°C (avoid light) |
| Detection Reagent B | 25 μL | -20°C (avoid light) |
| Enzyme Solution | 25 μL | -20°C (avoid light) |
| Fluorescent Detection Solution | 25 μL | -20°C (avoid light) |
| Positive Control | 25 μL | -20°C (avoid light) |
| Mineral Oil | 500 μL | -20°C (avoid light) |
| Extraction Solution | 5 mL | -20°C (avoid light) |
| Detection Tube | 24 tubes | room temperature |
| Extraction Tube | 24 tubes | room temperature |
The LAMP method is patented by Eiken Chemical Co., Ltd. Nippon Gene Co., Ltd. is licensed to develop, manufacture, and commercialize detection kits using the LAMP method.
* The linked page may be in Japanese, please use the translation function of the browser.